Journal: Applied Microbiology and Biotechnology

Article Title: Campycins are novel broad-spectrum antibacterials killing Campylobacter jejuni

doi: 10.1007/s00253-024-13317-w

Figure Lengend Snippet: H-fibers from CJIE1 prophages in C. jejuni strains CAMSA2147 and RM1221 and the construction of campycins. A Alignment of H-fibers from CJIE1 prophages present in CAMSA2147 (SAMN08987259) and RM1221 (SAMN14933898). Protein sequences were aligned using CLC Main Workbench 22 (QIAGEN), and gray boxes indicate divergent amino acids. The orange line indicates the amino acids present in campycins 1 and 2. B Schematic representation of campycins 1 and 2. Campycin 1 contains 164 amino acids of the N-terminus of Prf15 tail fiber originating from P . aeruginosa PAO1 R2-pyocin (gray) fused to 193 amino acids of the C-terminus of the H-fiber derived from the CAMSA2147 CJIE1 prophage (green) (10). Campycin 2 contains the N-terminus Prf15 encoded P. aeruginosa PAO1 R2-pyocin (gray) fused to 209 amino acids of the C-terminus of the H-fiber derived from the RM1221 CJIE1 prophage (blue)

Article Snippet: To investigate the diversity of CJIE1 H-fibers, CAMSA2147 H-fiber (WP_002878910.1) was used for searching CJIE1 H-fiber homologs in our CAMSA bacterial collection, and sequences were further aligned using CLC Main Workbench 22 (QIAGEN).

Techniques: Derivative Assay

Journal: Applied Microbiology and Biotechnology

Article Title: Campycins are novel broad-spectrum antibacterials killing Campylobacter jejuni

doi: 10.1007/s00253-024-13317-w

Figure Lengend Snippet: SDS-PAGE of pull-down assays with purified H-fibers and cell lysates from C. jejuni CAMSA2147 and RM1221. The C-terminus of the CAMSA2147 or RM1221 CJIE1 H-fibers were fused to a His-tag and co-expressed with the downstream chaperones. Purified H-fibers were used as bait in pull-down assays, and CAMSA2147 or RM1221 cell lysates as prey. The eluted fractions were analyzed by SDS-PAGE. A Pull-down assay with purified CAMSA2147 H-fiber. Lane 1, marker; lane 2, CAMSA2147 H-fiber co-expressed with the chaperone; lane 3, CAMSA2147 cell lysate; lane 4, CAMSA2147 cell lysate incubated with CAMSA2147 H-fiber co-expressed with the chaperone; lane 5, RM1221 cell lysate; lane 6, RM1221 cell lysate incubated with CAMSA2147 H-fiber co-expressed with the chaperone. ( B ) Pull-down assay with purified RM1221 H-fiber. Lane 1, marker; lane 2, RM1221 H-fiber co-expressed with the chaperone; lane 3, RM1221 cell lysate; lane 4, RM1221 cell lysate incubated with RM1221 H-fiber co-expressed with the chaperone; lane 5, CAMSA2147 cell lysates; lane 6, CAMSA2147 cell lysates incubated with RM1221 H-fiber co-expressed with the chaperone. Bands cut from the gel and sent for mass spectrometry analyses are indicated with an asterisk, and a full gel image is included in the supplementary material (Fig. )

Article Snippet: To investigate the diversity of CJIE1 H-fibers, CAMSA2147 H-fiber (WP_002878910.1) was used for searching CJIE1 H-fiber homologs in our CAMSA bacterial collection, and sequences were further aligned using CLC Main Workbench 22 (QIAGEN).

Techniques: SDS Page, Purification, Pull Down Assay, Marker, Incubation, Mass Spectrometry

Journal: Nanophotonics

Article Title: Contrast-enhanced near-infrared photoacoustic microscopy and optical coherence tomography imaging of rat fundus

doi: 10.1515/nanoph-2023-0872

Figure Lengend Snippet: Experimental setup of multimodal VIS/NIR-PAM-OCT system. (a) Illustration of the in vivo multimodal imaging using FINPs. The synthesized FINPs were intravenously injected into the rat model through vein. OCT and PAM signals from the rat fundus was generated by using superluminescent diodes (SLD) and nanosecond pulsed laser illumination at wavelength of either 532 or 1,064 nm. (b) Schematic diagram of the dual-modal imaging system. PC1, PC2, personal computer for scanning controlling and data acquisition; SLD, superluminescent diode; AT1, AT2, adjustable attenuator; BS, beam splitter; PD, photodiode; SMF, single mode fiber; HM1, HM2, hot mirror; GM, two-dimensional galvanometer; UT, ultrasonic transducer; M1, mirror; L1-L4, lens. (c) Physical setup of the dual-modal imaging system. 1,2-fiber coupled. 3-reference arm. 4-homebuilt spectrometer. 5,6,7-beam alignment.

Article Snippet: In the PAM system, near-infrared semiconductor lasers (MPL-H-1064; output wavelength: 1,064 nm; pulse energy: 19 μJ; pulse duration: 5 ns; pulse repetition rate: 5 kHz; Changchun New Industries Co., Ltd) and visible light semiconductor laser (MPL-H-532, output wavelength: 532 nm; pulse energy: 15 μJ; pulse duration: 4 ns; pulse repetition rate: 5 kHz; Changchun New Industries Co., Ltd) were used as the illumination sources for PAM imaging.

Techniques: In Vivo, Imaging, Synthesized, Injection, Generated